Open AccessLuminescent images of single gold nanoparticles and their labeling on silica beads
Author(s) -
Jian Zhang,
Yi Fu,
Joseph R. Lakowicz
Publication year - 2007
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.15.013415
Subject(s) - luminescence , fluorescence , materials science , nanoparticle , metal , confocal , nanotechnology , colloidal gold , microscopy , confocal microscopy , molecule , optics , chemistry , optoelectronics , organic chemistry , physics , metallurgy
Luminescent Au nanoparticles were synthesized in a modified Brust method (average diameters of metal core = 1.6 nm). The fluorescence images were measured using scanning confocal microscopy and validated as compared with organic fluorophores. The metal particles were functionalized with succinimidyl ester terminated ligands and bound as fluorophores on surface-aminated silica beads to mimic labeling of biological functionalities. The labeled silica beads were shown to display bright signals and good photostability. Our results indicate that the luminescent metal nanoparticles can be employed as the probes to label the biological functionalities in developing molecule imaging agents.