Open AccessProposal of three-dimensional phase contrast holographic microscopy
Author(s) -
Naoki Fukutake,
Tom D. Milster
Publication year - 2007
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.15.012662
Subject(s) - optics , digital holographic microscopy , holography , microscopy , digital holography , spatial frequency , phase contrast imaging , contrast (vision) , fourier transform , refractive index , phase (matter) , resolution (logic) , materials science , phase contrast microscopy , physics , computer science , artificial intelligence , quantum mechanics
We propose a three-dimensional phase contrast digital holographic microscopy. The object to be observed is a low-contrast transparent refractive index distribution sample, such as biological tissue. Low contrast phase objects are converted to high contrast images through the microscopy we propose. In order to gain high three-dimensional resolution, the direction of pump plane wave is scanned, and separate holographic images produced at each angle are acquired and decoded into complex amplitude in Fourier space. The three-dimensional image is reconstructed in a computer from all information acquired through the system. The resolution in the direction of the optical axis is increased by utilizing a 4pi configuration of objective lenzes.