Open AccessCoherent femtosecond pulse shaping for the optimization of a non-linear micro-endoscope
Author(s) -
Mickaël Lelek,
Eric Suran,
Frédéric Louradour,
Alain Barthélémy,
B. Viellerobe,
F. Lacombe
Publication year - 2007
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.15.010154
Subject(s) - optics , femtosecond , materials science , bundle , fiber bundle , compensation (psychology) , pulse shaping , dispersion (optics) , femtosecond pulse shaping , signal (programming language) , ultrashort pulse , laser , physics , computer science , psychology , psychoanalysis , composite material , programming language
A flexible multicore fiber bundle is fed by temporally and spectrally shaped femtosecond pulses allowing for the pre-compensation of both chromatic dispersion and non-linear effects encountered in the bundle. We demonstrate that the pulse duration at the fiber bundle output can be significantly reduced in comparison with linear pre-compensation only. The scheme for femtosecond pulse fiber delivery is applied to the optimization of two-photon fluorescence (TPF) imaging. Experiments and calculations show a five-fold improvement of the TPF signal produced at the end of the fiber bundle in comparison with linear pre-compensation. This is applied to the recording, in real time (12 image/s), of TPF laser-scanning images of human colon cells stained with a fluorescent marker. Further optimizations are discussed.