Open AccessMultidimensional non-linear laser imaging of Basal Cell Carcinoma
Author(s) -
Riccardo Cicchi,
Daniela Massi,
Serena Sestini,
Paolo Carli,
Vincenzo De Giorgi,
Torello Lotti,
Francesco S. Pavone
Publication year - 2007
Publication title -
optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.15.010135
Subject(s) - multispectral image , basal cell carcinoma , fluorescence lifetime imaging microscopy , fluorescence , optics , excitation wavelength , materials science , laser , two photon excitation microscopy , preclinical imaging , in vivo , basal cell , pathology , biology , medicine , physics , computer science , microbiology and biotechnology , computer vision
We have used a multidimensional non-linear laser imaging approach to visualize ex-vivo samples of basal cell carcinoma (BCC). A combination of several non-linear laser imaging techniques involving fluorescence lifetime, multispectral two-photon and second-harmonic generation imaging has been used to image different skin layers. This approach has elucidated some morphological (supported by histopathological images), biochemical, and physiochemical differences of the healthy samples with respect to BCC ones. In particular, in comparison with normal skin, BCC showed a blue-shifted fluorescence emission, a higher fluorescence response at 800 nm excitation wavelength and a slightly longer mean fluorescence lifetime. Finally, the use of aminolevulinic acid as a contrast agent has been demonstrated to increase the constrast in tumor border detection. The results obtained provide further support for in-vivo non-invasive imaging of Basal Cell Carcinoma.