Open AccessMicrophotonic control of single molecule fluorescence correlation spectroscopy using planar optofluidics
Author(s) -
Dongmei Yin,
Evan J. Lunt,
Anjan Barman,
Aaron R. Hawkins,
Holger Schmidt
Publication year - 2007
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.15.007290
Subject(s) - optofluidics , fluorescence correlation spectroscopy , planar , materials science , optics , photonics , spectroscopy , fluorescence spectroscopy , microscopy , lithography , fluorescence , optoelectronics , microfluidics , nanotechnology , physics , computer graphics (images) , quantum mechanics , computer science
We demonstrate the implementation of fluorescence correlation spectroscopy (FCS) on a chip. Full planar integration is achieved by lithographic definition of sub-picoliter excitation volumes using intersecting solid and liquid-core optical waveguides. Concentration dependent measurements on dye molecules with single molecule resolution are demonstrated. Theoretical modeling of the FCS autocorrelation function in microstructured geometries shows that the FCS behavior can be controlled over a wide range by tailoring the micro-photonic environment. The ability to perform correlation spectroscopy using silicon photonics without the need for free-space microscopy permits implementation of numerous diagnostic applications on compact planar optofluidic devices.