Open AccessDynamic disorder in horseradish peroxidase observed with total internal reflection fluorescence correlation spectroscopy
Author(s) -
Kaï Hassler,
Per Rigler,
Hans Blom,
Rudolf Rigler,
Jerker Widengren,
Theo Lasser
Publication year - 2007
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.15.005366
Subject(s) - total internal reflection fluorescence microscope , fluorescence correlation spectroscopy , horseradish peroxidase , total internal reflection , spectroscopy , optics , fluorescence , fluorescence spectroscopy , microscopy , materials science , reflection (computer programming) , nuclear magnetic resonance , analytical chemistry (journal) , chemistry , physics , programming language , quantum mechanics , chromatography , computer science , enzyme
This paper discusses the application of objective-type total internal reflection fluorescence correlation spectroscopy (TIR-FCS) to the study of the kinetics of immobilized horseradish peroxidase on a single molecule level. Objective-type TIR-FCS combines the advantages of FCS with TIRF microscopy in a way that allows for simultaneous ultra-sensitive spectroscopic measurements using a single-point detector and convenient localization of single molecules on a surface by means of parallel imaging.