Open AccessFibered confocal spectroscopy and multicolor imaging system for in vivo fluorescence analysis
Author(s) -
Florence Jean,
Geneviève Bourg-Heckly,
Bertrand Viellerobe
Publication year - 2007
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.15.004008
Subject(s) - confocal , optics , fluorescence lifetime imaging microscopy , materials science , preclinical imaging , confocal microscopy , microscope , fluorescence , microscopy , fluorescence microscope , spectroscopy , fluorescence spectroscopy , biomedical engineering , in vivo , physics , medicine , microbiology and biotechnology , quantum mechanics , biology
We report the design and implementation of spectroscopic and multicolor imaging capabilities into a fibered confocal fluorescence microscope (FCFM) already capable of in vivo imaging. The real time imaging device and the high resolution fiber probe make this system the first reported capable of performing multi color detection in the field of FCFM. The advantages of the system will allow in vivo morphological and functional imaging. Preliminary experiments were carried out in tissue samples to demonstrate the potential of the technique. The quality of the axial sectioning achieved in the confocal fluorescence spectroscopy mode is demonstrated experimentally, and applications to multicolor imaging are shown.