Quantitative phase microscopy with asynchronous digital holography | Zendy

Kevin J. Chalut | Zendy; William O. Brown | Zendy; Adam Wax | Zendy

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Quantitative phase microscopy with asynchronous digital holography

Author(s) -

Kevin J. Chalut,

William O. Brown,

Adam Wax

Publication year - 2007

Publication title -

optics express

Language(s) - Uncategorized

Resource type - Journals

SCImago Journal Rank - 1.394

H-Index - 271

ISSN - 1094-4087

DOI - 10.1364/oe.15.003047

Subject(s) - optics , digital holographic microscopy , digital holography , holography , phase imaging , interferometry , holographic interferometry , phase (matter) , asynchronous communication , microscopy , millisecond , sample (material) , computer science , phase retrieval , biological specimen , biological imaging , materials science , physics , fourier transform , computer network , quantum mechanics , astronomy , thermodynamics , fluorescence

We demonstrate a new method of measuring quantitative phase in imaging of biological materials. This method, asynchronous digital holography, employs knowledge of a moving fringe created by acousto-optic modulators to execute phase-shifting interferometry using two near-simultaneous interferograms. The method can be used to obtain quantitative phase images of dynamic biological samples on millisecond time scales. We present results on a standard sample, and on live cell samples.

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