Open AccessQuantitative phase microscopy with asynchronous digital holography
Author(s) -
Kevin J. Chalut,
William J. Brown,
Adam Wax
Publication year - 2007
Publication title -
optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.15.003047
Subject(s) - optics , digital holographic microscopy , digital holography , holography , phase imaging , interferometry , holographic interferometry , phase (matter) , asynchronous communication , microscopy , millisecond , sample (material) , computer science , phase retrieval , biological specimen , biological imaging , materials science , physics , fourier transform , computer network , quantum mechanics , astronomy , thermodynamics , fluorescence
We demonstrate a new method of measuring quantitative phase in imaging of biological materials. This method, asynchronous digital holography, employs knowledge of a moving fringe created by acousto-optic modulators to execute phase-shifting interferometry using two near-simultaneous interferograms. The method can be used to obtain quantitative phase images of dynamic biological samples on millisecond time scales. We present results on a standard sample, and on live cell samples.