Open AccessMicroscopic particle discrimination using spatially-resolved Fourier-holographic light scattering angular spectroscopy
Author(s) -
Timothy R. Hillman,
Sergey Alexandrov,
Thomas Gutzler,
David D. Sampson
Publication year - 2006
Publication title -
optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.14.011088
Subject(s) - optics , holography , scattering , mie scattering , light scattering , fourier transform , spectroscopy , microscopy , physics , characterization (materials science) , spatial frequency , fourier transform spectroscopy , multiangle light scattering , particle (ecology) , digital holographic microscopy , materials science , fourier transform infrared spectroscopy , quantum mechanics , oceanography , geology
We utilize Fourier-holographic light scattering angular spectroscopy to record the spatially resolved complex angular scattering spectra of samples over wide fields of view in a single or few image captures. Without resolving individual scatterers, we are able to generate spatially-resolved particle size maps for samples composed of spherical scatterers, by comparing generated spectra with Mie-theory predictions. We present a theoretical discussion of the fundamental principles of our technique and, in addition to the sphere samples, apply it experimentally to a biological sample which comprises red blood cells. Our method could possibly represent an efficient alternative to the time-consuming and laborious conventional procedure in light microscopy of image tiling and inspection, for the characterization of microscopic morphology over wide fields of view.