Open AccessIn-vivo NIR autofluorescence imaging of rat mammary tumors
Author(s) -
Laure Fournier,
V. Lucidi,
Kirill Berejnoi,
Theodore T. Miller,
Stavros G. Demos,
Robert C. Brasch
Publication year - 2006
Publication title -
optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.14.006713
Subject(s) - autofluorescence , in vivo , pathology , preclinical imaging , materials science , medicine , optics , fluorescence , biology , physics , microbiology and biotechnology
We investigate in vivo detection of mammary tumors in a rat model using autofluorescence imaging in the red and far-red spectral regions. The objective was to explore this method for non-invasive detection of malignant tumors and correlation between autofluorescence properties of tumors and their pathologic status. Eighteen tumor-bearing rats, bearing eight benign and seventeen malignant tumors were imaged. Autofluorescence images were acquired using spectral windows centered at 700-nm, 750-nm and 800-nm under laser excitation at 632.8-nm and 670- nm. Intensity in the autofluorescence images of malignant tumors under 670-nm excitation was higher than that of the adjacent normal tissue. whereas intensity of benign tumors was lower compared to normal tissue.