Open AccessReconstruction of complementary images in second harmonic generation microscopy
Author(s) -
Liang Gao,
Jin Liu,
Ping Xue,
Jun Xu,
Yi Wang,
Hui Ma,
Dieyan Chen
Publication year - 2006
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.14.004727
Subject(s) - image stitching , optics , microscopy , second harmonic generation , biological imaging , polarization (electrochemistry) , image processing , high harmonic generation , biological specimen , materials science , laser , computer science , computer vision , physics , image (mathematics) , chemistry , fluorescence
Second harmonic generation microscopy(SHGM) has become widely used to image biological samples. Due to the complexity of biological samples, more and more effort has been put on polarization imaging in SHGM technology to uncover their structures. In this work, we put forward a novel stitching method based on careful mathematical calculation, and accomplish it by rotating laser polarization. We first show its validity in imaging a perfectly synthesized bio-origin polymer poly (3-hyroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx). Then, we test its power by getting a true image of fibrillar collagen structure of rat-tail tendon.