Open AccessIn vivo nonlinear spectral imaging in mouse skin
Author(s) -
Jonathan A. Palero,
Henriëtte S. de Bruijn,
A. van der Ploeg–van den Heuvel,
Henricus J. C. M. Sterenborg,
Hans C. Gerritsen
Publication year - 2006
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.14.004395
Subject(s) - autofluorescence , optics , spectral imaging , microscopy , second harmonic generation , materials science , wavelength , imaging spectroscopy , medical imaging , spectroscopy , fluorescence , physics , laser , computer science , artificial intelligence , quantum mechanics
We report on two-photon autofluorescence and second harmonic spectral imaging of live mouse tissues. The use of a high sensitivity detector and ultraviolet optics allowed us to record razor-sharp deep-tissue spectral images of weak autofluorescence and short-wavelength second harmonic generation by mouse skin. Real-color image representation combined with depth-resolved spectral analysis enabled us to identify tissue structures. The results show that linking nonlinear deep-tissue imaging microscopy with autofluorescence spectroscopy has the potential to provide important information for the diagnosis of skin tissues.