Open AccessOptimization of metal-clad waveguides for sensitive fluorescence detection
Author(s) -
Aldo Minardo,
Romeo Bernini,
F. Mottola,
Luigi Zeni
Publication year - 2006
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.14.003512
Subject(s) - fluorescence , materials science , refractive index , optics , surface plasmon , substrate (aquarium) , microscope , laser induced fluorescence , optoelectronics , plasmon , oceanography , physics , geology
Recently, metal-clad leaky waveguides (MCLW) have been proposed as highly sensitive single point sensor devices for small-volume refractive index (RI) and fluorescence detection. In this paper, we present a theoretical study of the efficiency of MCLW-based sensors on glass substrate, for fluorescence detection. It is shown that MCLWs can be designed in order to obtain an efficient coupling of fluorescence emission with their leaky modes. This leads to a higher directionality of the fluorescence emission into the glass substrate, when compared to the emission near a pure glass/water interface and surface-plasmon coupled emission (SPCE). Numerical analyses also indicate that collecting the fluorescence emission through a water-immersed microscope objective, may result in a 70-fold enhancement of the detectable signal when compared to conventional fluorescence detection carried out on a glass slide.