Open AccessTwo-photon bioimaging with picosecond optical pulses from a semiconductor laser
Author(s) -
Hiroyuki Yokoyama,
Haotian Guo,
Takuya Yoda,
Keijiro Takashima,
Kimitoshi Sato,
Hiroki Taniguchi,
Hiromasa Ito
Publication year - 2006
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.14.003467
Subject(s) - optics , materials science , picosecond , optoelectronics , laser , semiconductor , photon , wavelength , pulse (music) , physics , detector
Toward a practical light source for two-photon bioimaging, we have generated kilowatt peak power of 0.77 mum wavelength and 5 ps optical pulse via second-harmonic generation of the amplified output from a gain-switched 1.55 mum semiconductor laser. This compact scheme and stable optical-pulse-source has been successfully used for the two-photon fluorescence bioimaging of actin filaments in PtK2 cells.