Open AccessTime-resolved spectrally constrained method for the quantification of chromophore concentrations and scattering parameters in diffusing media
Author(s) -
Cosimo D’Andrea,
Lorenzo Spinelli,
Andrea Bassi,
Arianna Giusto,
Davide Contini,
Johannes Swartling,
Alessandro Torricelli,
Rinaldo Cubeddu
Publication year - 2006
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.14.001888
Subject(s) - supercontinuum , optics , materials science , scattering , photon counting , light scattering , photonic crystal fiber , chromophore , photon diffusion , broadband , photon , optical fiber , physics , light source , quantum mechanics
We have devised and experimentally validated, on tissue-simulating phantoms and in vivo, a time-resolved spectral fitting analysis for direct assessment of chromophore concentrations and scattering parameters. Experimental data have been acquired with a time-resolved broadband system based on supercontinuum light generated in a photonic crystal fiber and a 32 channel Time Correlated Single Photon Counting system. The novel method is more robust than conventional techniques, especially at low signal-to-noise ratio.