
Determining nuclear morphology using an improved angle-resolved low coherence interferometry system
Author(s) -
John W. Pyhtila,
Robert N. Graf,
Adam Wax
Publication year - 2003
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.11.003473
Subject(s) - optics , interferometry , coherence (philosophical gambling strategy) , scattering , optical coherence tomography , calibration , materials science , light scattering , forward scatter , mie scattering , physics , quantum mechanics
We outline the process for determining the morphology of subsurface epithelial cell nuclei using depth-resolved light scattering measurements. The measurements are accomplished using a second generation angle-resolved low coherence interferometry system. The new system greatly improves data acquisition and analysis times compared to the initial prototype system. The calibration of the new system is demonstrated in scattering studies to determine the size distribution of polystyrene microspheres in a turbid sample. The process for determining the size of cell nuclei is discussed by analyzing measurements of basal cells in a sub-surface layer of intact, unstained epithelial tissue.