Open AccessA GRISM-based probe for spectrally encoded confocal microscopy
Author(s) -
Costas Pitris,
Brett E. Bouma,
Milen Shiskov,
Guillermo J. Tearney
Publication year - 2003
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.11.000120
Subject(s) - optics , confocal , grism , confocal microscopy , grating , materials science , microscope , microscopy , resolution (logic) , holography , diffraction grating , refractive index , physics , computer science , stars , astronomy , artificial intelligence , hubble space telescope
Spectrally encoded confocal microscopy (SECM) is a novel approach for obtaining high resolution, depth-sectioned images of microstructure within turbid samples. By encoding one spatial dimension in wavelength, imaging probes can be greatly simplified compared to standard scanning confocal microscopes, potentially enabling endoscopic implementation. The use of a diffraction grating for spectral encoding, however, skews the optical axis through the probe, thus complicating the design of narrow diameter instruments. In this Letter, we describe a novel use of a single-optical-axis element based on high index-of-refraction prisms and a transmission holographic grating for the design of narrow diameter SECM devices. Confocal images obtained with a 10.0 mm probe demonstrate a transverse resolution of 1.1 microm and a field of view of 650 microm.