Open AccessTheoretical versus empirical measures of retinal magnification for scaling AOSLO images
Author(s) -
Heather Heitkotter,
Alexander E Salmon,
Rachel E Linderman,
Jason Porter,
Joseph Carroll
Publication year - 2021
Publication title -
journal of the optical society of america. a, optics, image science, and vision./journal of the optical society of america. a, online
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.803
H-Index - 158
eISSN - 1520-8532
pISSN - 1084-7529
DOI - 10.1364/josaa.435917
Subject(s) - magnification , adaptive optics , retinal , optics , scale (ratio) , scaling , resolution (logic) , physics , artificial intelligence , computer science , mathematics , ophthalmology , medicine , geometry , quantum mechanics
The adaptive optics scanning light ophthalmoscope (AOSLO) allows cellular resolution imaging of the living retina. The accuracy of many quantitative measurements made from these images requires accurate estimates of the lateral scale of the images. Here, we used trial lenses, which are known to affect the relative magnification of the retinal image, to compare empirical measures of image scale with theoretical estimates from a four-surface optical model. The theoretical optical model overestimated the empirically determined change in image scale in 70% of the subjects examined, albeit to varying degrees. While the origin for the differences between subjects is not known, residual accommodation during imaging likely contributes to this variability in retinal magnification. These data provide an opportunity to derive improved lateral scaling error estimates for structural metrics extracted from AOSLO retinal images.