Open AccessHyperspectral wide-field time domain single-pixel diffuse optical tomography platform
Author(s) -
Qi Pian,
Ru Yao,
Xavier Intes
Publication year - 2018
Publication title -
biomedical optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.9.006258
Subject(s) - hyperspectral imaging , diffuse optical imaging , optics , digital micromirror device , detector , photomultiplier , photon counting , imaging phantom , pixel , image resolution , optical tomography , spectral imaging , computer science , tomography , physics , artificial intelligence
We present the design and comprehensive instrumental characterization of a time domain diffuse optical tomography (TD-DOT) platform based on wide-field illumination and wide-field hyperspectral time-resolved single-pixel detection for functional and molecular imaging in turbid media. The proposed platform combines two digital micro-mirror devices (DMDs) to generate structured light and a spectrally resolved multi-anode photomultiplier tube (PMT) detector in time domain for hyperspectral data acquisition over 16 wavelength channels based on the time-correlated single-photon counting (TCSPC) technique. The design of the proposed platform is described in detail and its characteristics in spatial, temporal and spectral dimensions are calibrated and presented. The performance of the system is further validated through a phantom study where two absorbers in glass tubes with spectral contrast are mapped in a turbid medium of ~20 mm thickness. The method presented here offers the potential of accelerating the imaging process and improving reconstruction results in TD-DOT and thus facilitates its wide spread use in preclinical and clinical in vivo imaging scenarios.