Open AccessSupercontinuum source-based multi-contrast optical coherence tomography for rat retina imaging
Author(s) -
Jia Pu Syu,
Waradee Buddhakosai,
ShihJen Chen,
Chang Chih Ke,
Shih Hwa Chiou,
Wen Chuan Kuo
Publication year - 2018
Publication title -
biomedical optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.9.006132
Subject(s) - supercontinuum , optical coherence tomography , optics , contrast (vision) , preclinical imaging , retinal , retina , fundus photography , fundus (uterus) , biomedical engineering , materials science , tomography , in vivo , medicine , ophthalmology , optical fiber , physics , fluorescein angiography , biology , microbiology and biotechnology , photonic crystal fiber
This study proposed an ultrahigh-resolution multi-contrast optical coherence tomography system integrated with fundus photography for in vivo retinal imaging of rodents. A supercontinuum light source was used in the system, providing an axial resolution of less than 3 µm within 1.8 mm (in the tissue). Three types of tissue contrast based on backscattered intensity, phase retardation, and microvasculature at a capillary level can be simultaneously obtained using the proposed system. Pigmented Long-Evans, non-pigmented (albino) Sprague Dawley, and Royal College of Surgeons rats were imaged and compared. In vivo imaging results were validated with histology.