Open AccessImproving resolution of second harmonic generation microscopy via scanning structured illumination
Author(s) -
Chia Hua Yeh,
Cheng Zn Tan,
Chung-Chih Cheng,
Jui Ting Hung,
Szu Yu Chen
Publication year - 2018
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.9.006081
Subject(s) - optics , microscopy , resolution (logic) , second harmonic generation , optical sectioning , superresolution , image resolution , materials science , computer science , physics , image (mathematics) , computer vision , artificial intelligence , laser
Second harmonic generation microscopy (SHGM) is a well-known technique for examining the noncentrosymmetric structures in biomedical research. However, without real-state transitions, fluorescence-based superresolution methods cannot be applied. To improve the resolution, fringe-scanning SHGM (FS-SHGM), which combines SHGM with structured illumination based on point-scanning, is introduced in this paper. The scanning path was modulated to generate illumination patterns. For the coherent parts of SHG signals, a mathematical model of image formation and reconstruction was established. Both simulations and experiments showed a resolution improvement factor of ~1.4 in the lateral and 1.56 in the axial directions for chicken tendons and mouse skin.