Improving resolution of second harmonic generation microscopy via scanning structured illumination | Zendy

Chia-Hua Yeh | Zendy; Cheng-Zn Tan | Zendy; Ching-Hsiao Arthur Cheng | Zendy; Jui-Ting Hung | Zendy; Szu-Yu Chen | Zendy

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Improving resolution of second harmonic generation microscopy via scanning structured illumination

Author(s) -

Chia-Hua Yeh,

Cheng-Zn Tan,

Ching-Hsiao Arthur Cheng,

Jui-Ting Hung,

Szu-Yu Chen

Publication year - 2018

Publication title -

biomedical optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.362

H-Index - 86

ISSN - 2156-7085

DOI - 10.1364/boe.9.006081

Subject(s) - optics , resolution (logic) , microscopy , second harmonic generation , superresolution , image resolution , optical sectioning , materials science , point spread function , computer science , image (mathematics) , physics , computer vision , artificial intelligence , laser

Second harmonic generation microscopy (SHGM) is a well-known technique for examining the noncentrosymmetric structures in biomedical research. However, without real-state transitions, fluorescence-based superresolution methods cannot be applied. To improve the resolution, fringe-scanning SHGM (FS-SHGM), which combines SHGM with structured illumination based on point-scanning, is introduced in this paper. The scanning path was modulated to generate illumination patterns. For the coherent parts of SHG signals, a mathematical model of image formation and reconstruction was established. Both simulations and experiments showed a resolution improvement factor of ~1.4 in the lateral and 1.56 in the axial directions for chicken tendons and mouse skin.

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