Open AccessSpatially confined quantification of bilirubin concentrations by spectroscopic visible-light optical coherence tomography
Author(s) -
C. N. Veenstra,
Wilma Petersen,
Ivo M. Vellekoop,
Wiendelt Steenbergen,
Nienke Bosschaart
Publication year - 2018
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.9.003581
Subject(s) - optical coherence tomography , optics , bilirubin , coherence (philosophical gambling strategy) , imaging phantom , biomedical engineering , medicine , materials science , physics , quantum mechanics
Spatially confined measurements of bilirubin in tissue can be of great value for noninvasive bilirubin estimations during neonatal jaundice, as well as our understanding of the physiology behind bilirubin extravasation. This work shows the potential of spectroscopic visible-light optical coherence tomography (sOCT) for this purpose. At the bilirubin absorption peak around 460 nm, sOCT suffers from a strong signal decay with depth, which we overcome by optimizing our system sensitivity through a combination of zero-delay acquisition and focus tracking. In a phantom study, we demonstrate the quantification of bilirubin concentrations between 0 and 650 µM with only a 10% difference to the expected value, thereby covering the entire clinical pathophysiological range.