Open AccessOn-chip light-sheet fluorescence imaging flow cytometry at a high flow speed of 1 m/s
Author(s) -
Taichi Miura,
Hideharu Mikami,
Akihiro Isozaki,
Takuro Ito,
Yasuyuki Ozeki,
Keisuke Goda
Publication year - 2018
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.9.003424
Subject(s) - materials science , fluorescence , microfluidics , microscopy , fluorescence microscope , microchannel , lab on a chip , fluorescence lifetime imaging microscopy , chip , cytometry , microfluidic chip , optics , flow cytometry , optoelectronics , nanotechnology , computer science , physics , telecommunications , biology , genetics
We present on-chip fluorescence imaging flow cytometry by light-sheet excitation on a mirror-embedded microfluidic chip. The method allows us to obtain microscopy-grade fluorescence images of cells flowing at a high speed of 1 m/s, which is comparable to the flow speed of conventional non-imaging flow cytometers. To implement the light-sheet excitation of flowing cells in a microchannel, we designed and fabricated a mirror-embedded PDMS-based microfluidic chip. To show its broad utility, we used the method to classify large populations of microalgal cells ( Euglena gracilis ) and human cancer cells (human adenocarcinoma cells). Our method holds promise for large-scale single-cell analysis.