On-chip light-sheet fluorescence imaging flow cytometry at a high flow speed of 1 m/s

We present on-chip fluorescence imaging flow cytometry by light-sheet excitation on a mirror-embedded microfluidic chip. The method allows us to obtain microscopy-grade fluorescence images of cells flowing at a high speed of 1 m/s, which is comparable to the flow speed of conventional non-imaging flow cytometers. To implement the light-sheet excitation of flowing cells in a microchannel, we designed and fabricated a mirror-embedded PDMS-based microfluidic chip. To show its broad utility, we used the method to classify large populations of microalgal cells ( Euglena gracilis ) and human cancer cells (human adenocarcinoma cells). Our method holds promise for large-scale single-cell analysis.