Open AccessNear-simultaneous quantification of glucose uptake, mitochondrial membrane potential, and vascular parameters in murine flank tumors using quantitative diffuse reflectance and fluorescence spectroscopy
Author(s) -
Caigang Zhu,
H Martin,
Brian T. Crouch,
Amy F. Martinez,
Martin Li,
Gregory M. Palmer,
Mark W. Dewhirst,
Nimmi Ramanujam
Publication year - 2018
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.9.003399
Subject(s) - fluorescence , biophysics , in vivo , glycolysis , metastasis , chemistry , fluorescence spectroscopy , metabolism , carbohydrate metabolism , chorioallantoic membrane , spectroscopy , photodynamic therapy , optical imaging , biochemistry , pathology , biology , cancer research , cancer , angiogenesis , medicine , optics , physics , microbiology and biotechnology , organic chemistry , quantum mechanics
The shifting metabolic landscape of aggressive tumors, with fluctuating oxygenation conditions and temporal changes in glycolysis and mitochondrial metabolism, is a critical phenomenon to study in order to understand negative treatment outcomes. Recently, we have demonstrated near-simultaneous optical imaging of mitochondrial membrane potential (MMP) and glucose uptake in non-tumor window chambers, using the fluorescent probes tetramethylrhodamine ethyl ester (TMRE) and 2-N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG). Here, we demonstrate a complementary technique to perform near-simultaneous in vivo optical spectroscopy of tissue vascular parameters, glucose uptake, and MMP in a solid tumor model that is most often used for therapeutic studies. Our study demonstrates the potential of optical spectroscopy as an effective tool to quantify the vascular and metabolic characteristics of a tumor, which is an important step towards understanding the mechanisms underlying cancer progression, metastasis, and resistance to therapies.