Open AccessWavefront division digital holographic microscopy
Author(s) -
Nimit Patel,
Vismay Trivedi,
Swapnil Mahajan,
Vani K. Chhaniwal,
Corinne Fournier,
Seonoh Lee,
Bahram Javidi,
Arun Anand
Publication year - 2018
Publication title -
biomedical optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.9.002779
Subject(s) - digital holographic microscopy , wavefront , holography , optics , microscope , digital holography , microscopy , holographic interferometry , depth of field , division (mathematics) , computer science , physics , mathematics , arithmetic
Digital holographic microscopy is the state of the art quantitative phase imaging of micro-objects including living cells. It is an ideal tool to image and quantify cell thickness profiles with nanometer thickness resolution. Digital holographic techniques usually are implemented using a two-beam setup that may be bulky and may not be field portable. Self-referencing techniques provide compact geometry but suffer from a reduction of the field of view. Here, we discuss the development of a wavefront division digital holographic microscope providing the full field of view with a compact system. The proposed approach uses a wavefront division module consisting of two lenses. The developed microscope is tested experimentally by measuring the physical and mechanical properties of red blood cells.