Open AccessCellular in vivo 3D imaging of the cornea by confocal laser scanning microscopy
Author(s) -
Sebastian Bohn,
Karsten Sperlich,
Stephan Allgeier,
Andreas Bartschat,
Ruby Kala Prakasam,
KlausMartin Reichert,
H. Stolz,
Rudolf Guthoff,
Ralf Mikut,
Bernd Köhler,
Oliver Stachs
Publication year - 2018
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.9.002511
Subject(s) - optics , confocal microscopy , microscopy , microscope , confocal , materials science , laser scanning , cornea , cuboid , laser , biomedical engineering , physics , medicine , geometry , mathematics
We present an in vivo confocal laser scanning microscopy based method for large 3D reconstruction of the cornea on a cellular level with cropped volume sizes up to 266 x 286 x 396 µm 3 . The microscope objective used is equipped with a piezo actuator for automated, fast and precise closed-loop focal plane control. Furthermore, we present a novel concave surface contact cap, which significantly reduces eye movements by up to 87%, hence increasing the overlapping image area of the whole stack. This increases the cuboid volume of the generated 3D reconstruction significantly. The possibility to generate oblique sections using isotropic volume stacks opens the window to slit lamp microscopy on a cellular level.