Open AccessWhite light polarization sensitive optical coherence tomography for sub-micron axial resolution and spectroscopic contrast in the murine retina
Author(s) -
Danielle J. Harper,
Marco Augustin,
Antonia Lichtenegger,
Pablo Eugui,
C. Reyes,
Martin Glösmann,
Christoph K. Hitzenberger,
Bernhard Baumann
Publication year - 2018
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.9.002115
Subject(s) - optics , supercontinuum , optical coherence tomography , retina , interferometry , materials science , polarization (electrochemistry) , image resolution , optical tomography , white light interferometry , laser , physics , wavelength , chemistry , photonic crystal fiber
A white light polarization sensitive optical coherence tomography system has been developed, using a supercontinuum laser as the light source. By detecting backscattered light from 400 - 700 nm, an axial resolution of 1.0 µm in air was achieved. The system consists of a free-space interferometer and two homemade spectrometers that detect orthogonal polarization states. Following system specifications, images of a healthy murine retina as acquired by this non-contact system are presented, showing high resolution reflectivity images as well as spectroscopic and polarization sensitive contrast. Additional images of the very-low-density-lipoprotein-receptor (VLDLR) knockout mouse model were acquired. The high resolution allows the detection of small lesions in the retina.