Open AccessAutomated spectroscopic retinal oximetry with visible-light optical coherence tomography
Author(s) -
Shaohua Pi,
Acner Camino,
William O. Cepurna,
Xiang Wei,
Miao Zhang,
David Huang,
John C. Morrison,
Yali Jia
Publication year - 2018
Publication title -
biomedical optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.9.002056
Subject(s) - optical coherence tomography , retinal , pulse oximetry , reproducibility , biomedical engineering , oxygen saturation , tomography , medicine , ophthalmology , optics , oxygen , chemistry , radiology , anesthesia , physics , organic chemistry , chromatography
Accurate, quantitative assessment of retinal blood oxygen saturation ( sO 2 ) may provide a useful early indicator of pathophysiology in several ocular diseases. Here, with visible-light optical coherence tomography (OCT), we demonstrate an automated spectroscopic retinal oximetry algorithm to measure the sO 2 within the retinal arteries (A- sO 2 ) and veins (V- sO 2 ) in rats by automatically detecting the vascular posterior boundary on cross-sectional structural OCT. The algorithm was validated in vitro with flow phantoms and in vivo in rats by comparing the sO 2 results, respectively, to those obtained using a blood gas analyzer and pulse oximetry. We also investigated the response of oxygen extraction (A-V sO 2 ), including inter-session reproducibility, at different inhaled oxygen concentrations.