Open AccessReflective lens-free imaging on high-density silicon microelectrode arrays for monitoring and evaluation of in vitro cardiac contractility
Author(s) -
Thomas Pauwelyn,
Richard Stahl,
Lakyn Mayo,
Xuan Zheng,
Andy Lambrechts,
Stefan Janssens,
Liesbet Lagae,
Veerle Reumers,
Dries Braeken
Publication year - 2018
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.9.001827
Subject(s) - microelectrode , biomedical engineering , cardiotoxicity , materials science , lens (geology) , contractility , multielectrode array , medicine , optics , chemistry , electrode , physics , chemotherapy
The high rate of drug attrition caused by cardiotoxicity is a major challenge for drug development. Here, we developed a reflective lens-free imaging (RLFI) approach to non-invasively record in vitro cell deformation in cardiac monolayers with high temporal (169 fps) and non-reconstructed spatial resolution (352 µm) over a field-of-view of maximally 57 mm 2 . The method is compatible with opaque surfaces and silicon-based devices. Further, we demonstrated that the system can detect the impairment of both contractility and fast excitation waves in cardiac monolayers. Additionally, the RLFI device was implemented on a CMOS-based microelectrode array to retrieve multi-parametric information of cardiac cells, thereby offering more in-depth analysis of drug-induced (cardiomyopathic) effects for preclinical cardiotoxicity screening applications.