Open AccessLabel-free light-sheet microfluidic cytometry for the automatic identification of senescent cells
Author(s) -
Meiai Lin,
Qiao Li,
Chao Liu,
Qiao Xu,
Changshun Shao,
Xuantao Su
Publication year - 2018
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.9.001692
Subject(s) - microfluidics , microfabrication , cytometry , light scattering , miniaturization , materials science , light sheet fluorescence microscopy , computer science , flow cytometry , optics , microscopy , nanotechnology , scattering , physics , medicine , alternative medicine , pathology , fabrication , scanning confocal electron microscopy , biology , genetics
Label-free microfluidic cytometry is of increasing interest for single cell analysis due to its advantages of high-throughput, miniaturization, as well as noninvasive detection. Here we develop a next generation label-free light-sheet microfluidic cytometer for single cell analysis by two-dimensional (2D) light scattering measurements. Our cytometer integrates light sheet illumination with a disposable hydrodynamic focusing unit, which can achieve 3D hydrodynamic focusing of a sample fluid to a diameter of 19 micrometer without microfabrication. This integration also improves the signal to noise ratio (SNR) for the acquisition of 2D light scattering patterns from label-free cells. Particle sizing with submicron resolution is achieved by our light-sheet flow cytometer, where Euclidean distance-based similarity measures are performed. Label-free, automatic classification of senescent and normal cells is achieved with a high accuracy rate by incorporating our light-sheet flow cytometry with support vector machine (SVM) algorithms. Our light-sheet microfluidic cytometry with a microfabrication-free hydrodynamic focusing unit may find wide applications for automatic and label-free clinical diagnosis.