Open AccessInterferometric temporal focusing microscopy using three-photon excitation fluorescence
Author(s) -
Keisuke Toda,
Keisuke Isobe,
Kamiki,
Hiroyuki Kawano,
Atsushi Miyawaki,
Katsumi Midorikawa
Publication year - 2018
Publication title -
biomedical optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.9.001510
Subject(s) - microscopy , two photon excitation microscopy , optics , fluorescence microscope , light sheet fluorescence microscopy , interferometry , materials science , fluorescence lifetime imaging microscopy , resolution (logic) , excitation , microscope , super resolution microscopy , photoactivated localization microscopy , excitation wavelength , fluorescence , image resolution , temporal resolution , optical microscope , physics , computer science , scanning electron microscope , artificial intelligence , quantum mechanics
Super-resolution microscopy has become a powerful tool for biological research. However, its spatial resolution and imaging depth are limited, largely due to background light. Interferometric temporal focusing (ITF) microscopy, which combines structured illumination microscopy and three-photon excitation fluorescence microscopy, can overcome these limitations. Here, we demonstrate ITF microscopy using three-photon excitation fluorescence, which has a spatial resolution of 106 nm at an imaging depth of 100 µm with an excitation wavelength of 1060 nm.