Open AccessHousehold light source for potent photo-dynamic antimicrobial effect and wound healing in an infective animal model
Author(s) -
Azeem Ullah,
Yuxiang Zhang,
Zafar Iqbal,
Yaxin Zhang,
Dong Wang,
Jincan Chen,
Ping Hu,
Zhuo Chen,
Mingdong Huang
Publication year - 2018
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.9.001006
Subject(s) - potency , antimicrobial , acinetobacter baumannii , photodynamic therapy , fluence , in vivo , photosensitizer , in vitro , pact , chemistry , materials science , biophysics , microbiology and biotechnology , biology , biochemistry , bacteria , photochemistry , pseudomonas aeruginosa , ion , history , genetics , organic chemistry , archaeology
Photodynamic antimicrobial chemotherapy (PACT) is considered a promising alternative to conventional antibiotic approach. We have previously developed a novel PS containing five lysine amino acids, pentalysine-β-carbonylphthalocyanine Zinc (ZnPc(Lys) 5 ), which in the presence of light, is highly toxic against a range of bacterial strains, including hospital isolated, drug resistant Acinetobacter baumannii. Here, we study the effect of light fluence of the two light sources on the PACT potency of ZnPc(Lys) 5 . We observed that an exposure of E.coli to a red LED light for only 2 seconds (light fluence of 0.15 J/cm 2 ) in the presence of ZnPc(Lys) 5 significantly eradicated 80% of the E.coli . We further demonstrated that a light fluence of 4.5 J/cm 2 from a household light source induced a noticeable photodynamic effect in vitro and in vivo animal model. This study points to a new research direction of reducing light illumination time by increasing potency of PS.