Imaging of pre-mRNA splicing in living subjects using a genetically encoded luciferase reporter | Zendy

Yaru Shi | Zendy; Wei Liu | Zendy; Haifeng Zheng | Zendy; Zhiqiang Li | Zendy; Xiaorui Shi | Zendy; Shixuan Cai | Zendy; ZhiQiang Jiao | Zendy; Wenjie Mao | Zendy; Jinrong Xie | Zendy; Jie Tian | Zendy; Fu Wang | Zendy

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Imaging of pre-mRNA splicing in living subjects using a genetically encoded luciferase reporter

Author(s) -

Yaru Shi,

Wei Liu,

Haifeng Zheng,

Zhiqiang Li,

Xiaorui Shi,

Shixuan Cai,

ZhiQiang Jiao,

Wenjie Mao,

Jinrong Xie,

Jie Tian,

Fu Wang

Publication year - 2018

Publication title -

biomedical optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.362

H-Index - 86

ISSN - 2156-7085

DOI - 10.1364/boe.9.000518

Subject(s) - rna splicing , luciferase , reporter gene , alternative splicing , messenger rna , biology , eukaryote , gene , gene expression , bioluminescence imaging , microbiology and biotechnology , computational biology , genetics , rna , genome , transfection

Pre-mRNA splicing is an essential step in gene expression in most eukaryote genes. Here we present the feasibility of a genetically encoded luciferase reporter to monitor the pre-mRNA splicing process in living cells and animals. We showed that the splicing activity change induced by isoginkgetin could be readily visualized in vitro both in a dose and time dependent manner. Moreover, the pre-mRNA splicing process could be also obviously detected in mice by bioluminescence imaging and confirmed by RT-PCR. Our work provided a reporter system that allows high-throughput screening of chemical libraries to identify potential compounds leading to aberrant patterns of splicing.

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