Open AccessHolographic line field en-face OCT with digital adaptive optics in the retina in vivo
Author(s) -
Laurin Ginner,
Tilman Schmoll,
Abhishek Kumar,
Matthias Salas,
Nastassia Pricoupenko,
Lara M. Wurster,
Rainer A. Leitgeb
Publication year - 2018
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.9.000472
Subject(s) - optics , optical coherence tomography , adaptive optics , holography , retina , physics , retinal , materials science , chemistry , biochemistry
We demonstrate a high-resolution line field en-face time domain optical coherence tomography (OCT) system using an off-axis holography configuration. Line field en-face OCT produces high speed en-face images at rates of up to 100 Hz. The high frame rate favors good phase stability across the lateral field-of-view which is indispensable for digital adaptive optics (DAO). Human retinal structures are acquired in-vivo with a broadband light source at 840 nm, and line rates of 10 kHz to 100 kHz. Structures of different retinal layers, such as photoreceptors, capillaries, and nerve fibers are visualized with high resolution of 2.8 µm and 5.5 µm in lateral directions. Subaperture based DAO is successfully applied to increase the visibility of cone-photoreceptors and nerve fibers. Furthermore, en-face Doppler OCT maps are generated based on calculating the differential phase shifts between recorded lines.