Open AccessOptical volumetric projection for fast 3D imaging through circularly symmetric pupil engineering
Author(s) -
Bo Cai,
Xiaofan Zhai,
Zeguan Wang,
Yongming Shen,
Ronald X. Xu,
Zachary J. Smith,
Quan Wen,
Kaiqin Chu
Publication year - 2018
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.9.000437
Subject(s) - point spread function , optics , projection (relational algebra) , position (finance) , microscopy , depth of field , phase retrieval , image resolution , physics , optical imaging , light field , materials science , computer science , fourier transform , finance , algorithm , quantum mechanics , economics
Monitoring and manipulating neuronal activities with optical microscopy desires a method where light can be focused or projected over a long axial range so that large brain tissues (>100 [Formula: see text] thick) can be simultaneously imaged, and specific brain regions can be optogenetically stimulated without the need for slow optical refocusing. However, the micron-scale resolution required in neuronal imaging yields a depth of field of less than 10 [Formula: see text] in conventional imaging systems. We propose to use a circularly symmetric phase mask to extend the depth of field. A numerical study shows that our method maintains both the peak and the shape of the point spread function vs the axial position better than current methods. Imaging of a 3D bead suspension and sparsely labelled thick brain tissue confirms the feasibility of the system for fast volumetric imaging.