Open AccessMultispectral photoacoustic microscopy of lipids using a pulsed supercontinuum laser
Author(s) -
Takashi Buma,
Nicole C. Conley,
Sang Won Choi
Publication year - 2017
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.9.000276
Subject(s) - supercontinuum , microscopy , materials science , laser , optics , multispectral image , absorption (acoustics) , fiber laser , microscope , photonic crystal fiber , photoacoustic imaging in biomedicine , resolution (logic) , optoelectronics , remote sensing , physics , wavelength , computer science , artificial intelligence , geology
We demonstrate optical resolution photoacoustic microscopy (OR-PAM) of lipid-rich tissue between 1050-1714 nm using a pulsed supercontinuum laser based on a large-mode-area photonic crystal fiber. OR-PAM experiments of lipid-rich samples show the expected optical absorption peaks near 1210 and 1720 nm. These results show that pulsed supercontinuum lasers are promising for OR-PAM applications such as label-free histology of lipid-rich tissue and imaging small animal models of disease.