Open AccessHigh-throughput imaging of zebrafish embryos using a linear-CCD-based flow imaging system
Author(s) -
Lifeng Liu,
Guang Yang,
Shoupeng Liu,
Linbo Wang,
Yang Xiao-yan,
Hanbing Qu,
Xiaofen Liu,
Liqin Cao,
Weijun Pan,
Hui Li
Publication year - 2017
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.8.005651
Subject(s) - zebrafish , embryo , computer science , image processing , throughput , live cell imaging , medical imaging , light sheet fluorescence microscopy , artificial intelligence , computer vision , biology , microscopy , optics , image (mathematics) , microbiology and biotechnology , physics , cell , telecommunications , biochemistry , scanning confocal electron microscopy , gene , wireless , genetics
High-throughput imaging and screening is essential for biomedical research and drug discovery using miniature model organisms such as zebrafish. This study introduces a high-speed imaging system which illuminates zebrafish embryos flowing through a capillary tube with a sheet of light and captures them using a linear charge-coupled device (CCD). This system can image dozens of zebrafish embryos per second. An image algorithm was developed to recognize each embryo and to perform automatic analysis. We distinguished dead and living embryos according to the gray level distribution and conducted statistics of morphological characteristics of embryos at different growing stages.