Open AccessLabel-free sensing of the binding state of MUC1 peptide and anti-MUC1 aptamer solution in fluidic chip by terahertz spectroscopy
Author(s) -
Xiang Zhao,
Mingkun Zhang,
Dongshan Wei,
Yunxia Wang,
Shihan Yan,
Mengwan Liu,
Yang Xiang,
Ke Yang,
HongLiang Cui,
Weiling Fu
Publication year - 2017
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.8.004427
Subject(s) - aptamer , terahertz radiation , hydrogen bond , fluidics , terahertz spectroscopy and technology , muc1 , spectroscopy , molecule , materials science , absorption (acoustics) , absorption spectroscopy , nanotechnology , chemical physics , analytical chemistry (journal) , chemistry , optoelectronics , optics , mucin , physics , chromatography , organic chemistry , biochemistry , genetics , quantum mechanics , engineering , composite material , biology , aerospace engineering
The aptamer and target molecule binding reaction has been widely applied for construction of aptasensors, most of which are labeled methods. In contrast, terahertz technology proves to be a label-free sensing tool for biomedical applications. We utilize terahertz absorption spectroscopy and molecular dynamics simulation to investigate the variation of binding-induced collective vibration of hydrogen bond network in a mixed solution of MUC1 peptide and anti-MUC1 aptamer. The results show that binding-induced alterations of hydrogen bond numbers could be sensitively reflected by the variation of terahertz absorption coefficients of the mixed solution in a customized fluidic chip. The minimal detectable concentration is determined as 1 pmol/μL, which is approximately equal to the optimal immobilized concentration of aptasensors.