Open AccessOptical imaging for the assessment of hepatocyte metabolic state in ischemia and reperfusion injuries
Author(s) -
Mette Funding la Cour,
Shima Mehrvar,
Joohyun Kim,
A. Daniel Martin,
Michael A. Zimmerman,
Johnny C. Hong,
Mahsa Ranji
Publication year - 2017
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.8.004419
Subject(s) - ischemia , oxidative stress , hepatocyte , redox , reperfusion injury , mitochondrion , oxidative phosphorylation , liver transplantation , medicine , cofactor , chemistry , transplantation , pathology , andrology , biochemistry , enzyme , organic chemistry , in vitro
Deterioration in mitochondrial function leads to hepatic ischemia and reperfusion injury (IRI) in liver surgery and transplantation. 3D optical cryoimaging was used to measure the levels of mitochondrial coenzymes NADH and FAD, and their redox ratio (NADH/FAD) gave a quantitative marker for hepatocyte oxidative stress during IRI. Using a rat model, five groups were compared: control, ischemia for 60 or 90 minutes (Isc60, Isc90), ischemia for 60 or 90 minutes followed by reperfusion of 24 hours (IRI60, IRI90). Ischemia alone did not cause a significant increase in the redox ratio; however, the redox ratio in both IRI60 and IRI90 groups was significantly decreased by 29% and 71%, respectively. A significant correlation was observed between the redox ratio and other markers of injury such as serum aminotransferase levels and the tissue ATP level. The mitochondrial redox state can be successfully measured using optical cryoimaging as a quantitative marker of hepatic IR injury.