Open AccessLine scanning, stage scanning confocal microscope (LSSSCM)
Author(s) -
Daniel S. Gareau,
James G. Krueger,
Jason E. Hawkes,
Samantha R. Lish,
Michael P Dietz,
Alba Guembe Mülberger,
Euphemia W. Mu,
Mary L. Stevenson,
John M. Lewin,
Shane A Meehan,
John A. Carucci
Publication year - 2017
Publication title -
biomedical optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.8.003807
Subject(s) - optics , confocal , microscope , materials science , optical sectioning , confocal microscopy , cardinal point , lens (geology) , laser scanning , scanning confocal electron microscopy , light sheet fluorescence microscopy , microscopy , perpendicular , resolution (logic) , laser , physics , computer science , geometry , mathematics , artificial intelligence
For rapid pathological assessment of large surgical tissue excisions with cellular resolution, we present a line scanning, stage scanning confocal microscope (LSSSCM). LSSSCM uses no scanning mirrors. Laser light is focused with a single cylindrical lens to a line of diffraction-limited width directly into the (Z) sample focal plane, which is parallel to and near the flattened specimen surface. Semi-confocal optical sections are derived from the linear array distribution (Y) and a single mechanical drive that moves the sample parallel to the focal plane and perpendicular to the focused line (X). LSSSCM demonstrates cellular resolution in the conditions of high nuclear density within micronodular basal cell carcinoma.