Open AccessFluorescence depth estimation from wide-field optical imaging data for guiding brain tumor resection: a multi-inclusion phantom study
Author(s) -
Dennis J. Wirth,
Kolbein Kolste,
Stephen C. Kanick,
David W. Roberts,
Frédéric Leblond,
Keith D. Paulsen
Publication year - 2017
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.8.003656
Subject(s) - fluorescence , optics , materials science , fluorophore , imaging phantom , fluorescence lifetime imaging microscopy , alexa fluor , laser , collimated light , optical coherence tomography , biomedical engineering , physics , medicine
Studies have shown that fluorescent agents demarcate tumor from surrounding brain tissue and offer intraoperative guidance during resection. However, visualization of fluorescence signal from tumor below the surgical surface or through the appearance of blood in the surgical field is challenging. We have previously described red light imaging techniques for estimating fluorescent depths in turbid media. In this study, we evaluate these methods over a broader range of fluorophore concentrations, and investigate the ability to resolve multiple fluorescent emissions in the same plane or at different depths along the axis of imaging. A tungsten halogen lamp is used as a broadband white light source for reflectance imaging. Fluorescence from Alexa Fluor 647 is excited with a 635 nm diode laser. Reflectance and fluorescence spectral data are gathered between 670 and 720 nm with the use of a liquid crystal tunable filter and recorded on a sCMOS camera. Results show that two fluorescent emissions can be resolved within 2 mm if they are in the same plane or within 3 mm if they are at different depths along the axis of imaging up to 6 mm below the surface.