Open AccessDetection of rotavirus in clinical specimens using an immunosensor prototype based on the photon burst counting technique
Author(s) -
Makoto Hasegawa,
Ernest Apondi Wandera,
Y. Inoue,
Kimura Nanami,
Ryuzo Sasaki,
Tamio Mizukami,
Mohammad Monir Shah,
Nobuaki Shirai,
Osamu Takei,
Hironori Shindo,
Yuki Ichinose
Publication year - 2017
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.8.003383
Subject(s) - rotavirus , fluorescence , photon counting , acute gastroenteritis , confocal , gold standard (test) , materials science , optics , virology , virus , photon , physics , medicine
In this study, a sensitive fluorescence sensor was developed for the detection of small, fluorescence-labeled particles dispersed in a solution. The prototype system comprises of a laser confocal optical system and a mechanical sample stage to detect photon bursting of fluorescence-labeled small particles in sample volumes less than 5 μL within 3 minutes. To examine the feasibility of the prototype system as a diagnostic tool, assemblages of rotavirus and fluorescence-labeled antibody were analyzed. The detection sensitivity for rotavirus was 1 × 10 4 pfu/mL. Rotavirus in stool samples from patients with acute gastroenteritis was also detected. The advantages and disadvantages of this immunosensor with respect to ELISA and RT-PCR, the current gold standards for virus detection, are discussed.