Open AccessMultiphoton microscopy for label-free identification of intramural metastasis in human esophageal squamous cell carcinoma
Author(s) -
Jian Xu,
Deyong Kang,
Yaping Zeng,
Shuangmu Zhuo,
Xiaodong Zhu,
Liwei Jiang,
Jianxin Chen,
Jiangbo Lin
Publication year - 2017
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.8.003360
Subject(s) - multiphoton fluorescence microscope , microscopy , esophageal squamous cell carcinoma , metastasis , endoscope , fluorescence microscope , pathology , fluorescence lifetime imaging microscopy , fluorescence , materials science , medicine , biomedical engineering , carcinoma , optics , radiology , cancer , physics
For complete removal of cancerous tissue in esophageal squamous cell carcinoma (ESCC), intramural metastasis (IM) should be identified preoperatively or intraoperatively. Here, multiphoton microscopy (MPM) was introduced for label-free identification of IM in the esophageal wall, by a combination of two-photon excited fluorescence (TPEF), second harmonic generation (SHG) imaging, and spectral analysis. Three-dimensional (3D) imaging of the IM region was also performed. Quantitative parameters, including 3D fiber orientation, were measured by 3D-weighted orientation vector summation. Overall, MPM showed the potential to identify IM. With the development of the advanced MPM endoscope, clinical identification of IM by MPM will be possible.