Open AccessLooking inside the heart: a see-through view of the vascular tree
Author(s) -
Imke Nehrhoff,
Jorge Ripoll,
Rafael Samaniego,
Manuel Desco,
Marı́a Victoria Gómez-Gaviro
Publication year - 2017
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.8.003110
Subject(s) - visualization , computer science , tree (set theory) , biomedical engineering , pathology , computer vision , medicine , artificial intelligence , mathematical analysis , mathematics
The ability to acquire 3D images of the heart and its vasculature at cellular resolution facilitates a more detailed study of many heart diseases. Here, we describe a novel technique to image in 3D the heart vasculature by combining the CUBIC clearing protocol combined with in vivo administration of fluorescent-labeled lectin. The use of these techniques in combination with S elective P lane I llumination M icroscopy (SPIM) made it possible to obtain high resolution 3D images of the cardiac vascular tree. This methodological approach may enhance the visualization of 3D images of the cardiac vasculature remodeling associated with coronary disease.