Open AccessLine-scanning hyperspectral imaging based on structured illumination optical sectioning
Author(s) -
Yu John Hsu,
Chih Chiang Chen,
Chien Chung Huang,
Chia Hua Yeh,
Li Ying Liu,
Szu Yu Chen
Publication year - 2017
Publication title -
biomedical optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.8.003005
Subject(s) - hyperspectral imaging , optics , spectral imaging , optical sectioning , materials science , resolution (logic) , full spectral imaging , image resolution , focus (optics) , fluorescence , contrast (vision) , biomedical engineering , remote sensing , computer science , physics , artificial intelligence , medicine , geology
Line-scanning hyperspectral imaging (LHSI) is known to have a higher acquisition rate but lower sectioning capability than point-scanning hyperspectral imaging. To further increase the axial imaging contrast of LHSI, structured illumination was integrated into line excitation to remove the off-focus and scattered on-focus fluorescence signals. In an unsectioned leaf, the imaging contrast can be enhanced by 8 times, while in sectioned mouse skin tissues, a 4.5-fold enhancement can be achieved. With a spectral resolution of 1.15 nm, the fluorophores with seriously-overlapped spectra was proved to be separated without cross-talk by applying linear unmixing to the recorded spectral information.