Open AccessEnhancement of short coherence digital holographic microscopy by optical clearing
Author(s) -
Zhiyuan Shen,
Xiaorui Guo,
Yilong Zhang,
Dongmei Li,
Yonghong He
Publication year - 2017
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.8.002036
Subject(s) - optical coherence tomography , optics , holography , imaging phantom , microscopy , biomedical engineering , digital holography , digital holographic microscopy , coherence (philosophical gambling strategy) , materials science , image quality , optical microscope , computer science , medicine , physics , artificial intelligence , scanning electron microscope , quantum mechanics , image (mathematics)
In this work, we used a short coherence digital holographic microscopy system to demonstrate cross-talk noise suppression and imaging performance enhancement by optical clearing. Performance of the system on both phantom and in vitro porcine skin tissues before and after the treatment of 70% v./v. glycerol-saline solution was investigated. Our results showed that optical clearing effectively inhibits the cross-talk noise and improves the image quality in the deep of the in vitro porcine skin tissues. The imaging depth was increased by about 30% after topical application of the glycerol-saline solution for 30 min.