Open AccessClinically compatible flexible wide-field multi-color fluorescence endoscopy with a porcine colon model
Author(s) -
Gyugnseok Oh,
Youngrong Park,
Su Woong Yoo,
Soonjoo Hwang,
Alexey V. Dan Chin-Yu,
YeonMi Ryu,
SangYeob Kim,
Eunju Do,
Ki Hean Kim,
Sungjee Kim,
SeungJae Myung,
Euiheon Chung
Publication year - 2017
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.8.000764
Subject(s) - molecular imaging , ex vivo , in vivo , fluorescence , colorectal cancer , pathology , endoscopy , preclinical imaging , fluorescence lifetime imaging microscopy , medicine , biomedical engineering , radiology , cancer , biology , optics , physics , microbiology and biotechnology
Early detection of structural or molecular changes in dysplastic epithelial tissues is crucial for cancer screening and surveillance. Multi-targeting molecular endoscopic fluorescence imaging may improve noninvasive detection of precancerous lesions in the colon. Here, we report the first clinically compatible, wide-field-of-view, multi-color fluorescence endoscopy with a leached fiber bundle scope using a porcine model. A porcine colon model that resembles the human colon is used for the detection of surrogate tumors composed of multiple biocompatible fluorophores (FITC, ICG, and heavy metal-free quantum dots (hfQDs)). With an ex vivo porcine colon tumor model, molecular imaging with hfQDs conjugated with MMP14 antibody was achieved by spraying molecular probes on a mucosa layer that contains xenograft tumors. With an in vivo porcine colon embedded with surrogate tumors, target-to-background ratios of 3.36 ± 0.43, 2.70 ± 0.72, and 2.10 ± 0.13 were achieved for FITC, ICG, and hfQD probes, respectively. This promising endoscopic technology with molecular contrast shows the capacity to reveal hidden tumors and guide treatment strategy decisions.