Open AccessMultifrequency-swept optical coherence microscopy for highspeed full-field tomographic vibrometry in biological tissues
Author(s) -
Samuel Choi,
Keita Sato,
T. Ôta,
Fumiaki Nin,
Shogo Muramatsu,
Hiroshi Hibino
Publication year - 2017
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.8.000608
Subject(s) - optical coherence tomography , optics , laser doppler velocimetry , microscopy , coherence (philosophical gambling strategy) , doppler effect , heterodyne (poetry) , tomography , tomographic reconstruction , materials science , physics , acoustics , medicine , blood flow , quantum mechanics , astronomy
Because conventional laser Doppler vibrometry or Doppler optical coherence tomography require mechanical scanning probes that cannot simultaneously measure the wide-range dynamics of bio-tissues, a multifrequency-swept optical coherence microscopy with wide-field heterodyne detection technique was developed. A 1024 × 1024 × 2000 voxel volume was acquired with an axial resolution of ~1.8 μm and an acquisition speed of 2 s. Vibration measurements at 10 kHz were performed over a wide field of view. Wide-field tomographic vibration measurements of a mouse tympanic membrane are demonstrated to illustrate the applicability of this method to live animals.