Open AccessCompact diode laser source for multiphoton biological imaging
Author(s) -
Robert D. Niederriter,
Baris N. Ozbay,
Gregory L. Futia,
Emily A. Gibson,
Juliet T. Gopinath
Publication year - 2016
Publication title -
biomedical optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.8.000315
Subject(s) - laser , optics , materials science , optoelectronics , penetration depth , pulse duration , microscopy , laser diode , wavelength , laser power scaling , microscope , diode , fiber laser , fluorescence , semiconductor laser theory , physics
We demonstrate a compact, pulsed diode laser source suitable for multiphoton microscopy of biological samples. The center wavelength is 976 nm, near the peak of the two-photon cross section of common fluorescent markers such as genetically encoded green and yellow fluorescent proteins. The laser repetition rate is electrically tunable between 66.67 kHz and 10 MHz, with 2.3 ps pulse duration and peak powers >1 kW. The laser components are fiber-coupled and scalable to a compact package. We demonstrate >600 μm depth penetration in brain tissue, limited by laser power.