Open AccessStatistical parametric mapping of stimuli evoked changes in total blood flow velocity in the mouse cortex obtained with extended-focus optical coherence microscopy
Author(s) -
Paul J. Marchand,
Arno Bouwens,
Tristan Bolmont,
Vincent Khashayar Shamaei,
David Nguyen,
Daniel Szlag,
Jérôme Extermann,
Theo Lasser
Publication year - 2016
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.8.000001
Subject(s) - optical coherence tomography , statistical parametric mapping , blood flow , functional magnetic resonance imaging , microscopy , coherence (philosophical gambling strategy) , cerebral blood flow , optics , temporal resolution , biomedical engineering , repeatability , neuroimaging , hemodynamics , image resolution , materials science , neuroscience , magnetic resonance imaging , physics , chemistry , medicine , biology , cardiology , radiology , chromatography , quantum mechanics
Functional magnetic resonance (fMRI) imaging is the current gold-standard in neuroimaging. fMRI exploits local changes in blood oxygenation to map neuronal activity over the entire brain. However, its spatial resolution is currently limited to a few hundreds of microns. Here we use extended-focus optical coherence microscopy (xfOCM) to quantitatively measure changes in blood flow velocity during functional hyperaemia at high spatio-temporal resolution in the somatosensory cortex of mice. As optical coherence microscopy acquires hundreds of depth slices simultaneously, blood flow velocity measurements can be performed over several vessels in parallel. We present the proof-of-principle of an optimised statistical parametric mapping framework to analyse quantitative blood flow timetraces acquired with xfOCM using the general linear model. We demonstrate the feasibility of generating maps of cortical hemodynamic reactivity at the capillary level with optical coherence microscopy. To validate our method, we exploited 3 stimulation paradigms, covering different temporal dynamics and stimulated limbs, and demonstrated its repeatability over 2 trials, separated by a week.